主要功能
成像功能:對(duì) Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 種參數(shù)進(jìn)行成像分析。測(cè)定調(diào)節(jié)性能量耗散 Y(NPQ),反映植物光保護(hù)能力,測(cè)定非調(diào)節(jié)性能量耗散Y(NO),反映植物光損傷程度。
程序測(cè)量功能:可程序測(cè)量熒光誘導(dǎo)曲線、快速光曲線和暗弛豫,也可手動(dòng)測(cè)量;在測(cè)量過程中能自動(dòng)分析所有熒光參數(shù)的變化趨勢(shì)
AOI 功能:可在測(cè)量前或測(cè)量后任意選擇感興趣的區(qū)域(AOI),程序?qū)⒆詣?dòng)對(duì)選擇的 AOI 的數(shù)據(jù)進(jìn)行變化趨勢(shì)分析,并在報(bào)告文件中顯示相關(guān) AOI 的數(shù)據(jù)。所有報(bào)告文件中顯示的數(shù)據(jù)都可導(dǎo)出到 EXCEL 文件中。
成像異質(zhì)性分析功能:對(duì)任意參數(shù)任意時(shí)間的成像,可在圖像上任意選取兩點(diǎn),軟件自動(dòng)對(duì)兩點(diǎn)間的數(shù)據(jù)進(jìn)行橫向異質(zhì)性分析,并可導(dǎo)出到 EXCEL 文件中。
成像數(shù)據(jù)范圍分析功能:對(duì)任意參數(shù)任意時(shí)間的成像,可分析任意兩個(gè)熒光數(shù)值之間有多少個(gè)像素點(diǎn),多少面積(cm2)。
突變株篩選功能:可跟據(jù)成像結(jié)果快速篩選光合、產(chǎn)氫/油、抗逆(抗鹽、抗旱、抗病等)等突變株。
微藻毒理研究功能:可同時(shí)測(cè)量 96 個(gè)微藻樣品(對(duì)照和處理組)的光合活性,軟件自動(dòng)給出處理組樣品相對(duì)于對(duì)照組的光合抑制百分比。
吸光系數(shù)測(cè)量功能:快速測(cè)量葉片的吸光系數(shù)。吸光系數(shù)測(cè)量光源: 16 個(gè)紅光(650 nm)和 16 個(gè)近紅外(780 nm)LED,用于測(cè)量植物葉片或藻類樣品 PAR 吸光系數(shù)。
光強(qiáng)異質(zhì)性:測(cè)量區(qū)域光強(qiáng)異質(zhì)性≤±2%
測(cè)量參數(shù)
Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。
應(yīng)用領(lǐng)域
光合作用研究:可以在完全相同的條件下同時(shí)對(duì)大量樣品進(jìn)行成像
植物病理學(xué):病斑部位(包括肉眼不可見時(shí))成像以及病斑擴(kuò)散的時(shí)空動(dòng)力學(xué)
植物脅迫生 理學(xué):肉眼不可見脅迫損傷的早期檢測(cè)
遺傳育種:出苗后大規(guī)??焖俸Y選高光合/抗旱/抗熱/抗凍/抗病等植株
突變株篩選:快速篩選模式植物的光合突變株、抗逆突變株、產(chǎn)氫微藻突變株等
微藻毒理學(xué):不同毒物濃度多個(gè)重復(fù)的樣品一次測(cè)完,軟件自動(dòng)計(jì)算抑制比率
分子生物學(xué):宏觀水平上檢測(cè)樣品的綠色熒光蛋白(GFP)熒光
其它多種擴(kuò)展研究
選購(gòu)指南
一、熒光成像 MAXI 版
系統(tǒng)組成:通用型主機(jī),LED 供電單元, LED 光源,CCD,數(shù)據(jù)線,軟件等。
注意:高等植物或真核藻類測(cè)量時(shí)選擇藍(lán)光光源(推薦),藍(lán)藻測(cè)量請(qǐng)選擇紅光光源。
熒光成像 MAXI 版本 |
二、熒光成像 MINI 版
系統(tǒng)組成:通用型主機(jī)(下圖中未顯示),LED 光源(如下圖),CCD,數(shù)據(jù)線,軟件等。
注意:高等植物或真核藻類測(cè)量時(shí)選擇藍(lán)光光源(推薦),藍(lán)藻測(cè)量請(qǐng)選擇紅光光源。
熒光成像 MINI 版 |
三、熒光成像 MICROSCOPY 版
系統(tǒng)組成:通用型主機(jī),特制顯微鏡,CCD,數(shù)據(jù)線,軟件等。
熒光成像 MICROSCOPY 版 |
葡萄葉片 Y(NPQ) 成像 | 葡萄葉片 PS/50 成像結(jié)果 | MINI 版本成像結(jié)果 |
| | |
顯微版本成像結(jié)果 | 軟件成像界面 | 慢速誘導(dǎo)動(dòng)力學(xué)曲線 |
產(chǎn)地:德國(guó) WALZ
參考文獻(xiàn)
數(shù)據(jù)來源:光合作用文獻(xiàn) Endnote 數(shù)據(jù)庫(kù),更新至 2021 年 1 月,文獻(xiàn)數(shù)量超過 10000 篇
原始數(shù)據(jù)來源:Google Scholar
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